Inactivated SARS-CoV vaccine prepared from whole virus induces a high level of neutralizing antibodies in BALB/c mice.
Identifieur interne : 005292 ( Main/Exploration ); précédent : 005291; suivant : 005293Inactivated SARS-CoV vaccine prepared from whole virus induces a high level of neutralizing antibodies in BALB/c mice.
Auteurs : Lin Tang [République populaire de Chine] ; Qingyu Zhu ; Ede Qin ; Man Yu ; Zhifen Ding ; Huiying Shi ; Xiaojie Cheng ; Caiping Wang ; Guohui Chang ; Qiang Zhu ; Fang Fang ; Haiyan Chang ; Shuangli Li ; Xumin Zhang ; Xishu Chen ; Jun Yu ; Jian Wang ; Ze ChenSource :
- DNA and cell biology [ 1044-5498 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- Aluminum Hydroxide, Animals, Antibodies, Viral (immunology), Chlorocebus aethiops, Chromatography, Enzyme-Linked Immunosorbent Assay, Female, Immune Sera (immunology), Mice, Mice, Inbred BALB C, Propiolactone, SARS Virus (immunology), Vaccines, Inactivated (immunology), Vero Cells, Viral Vaccines (immunology).
- MESH :
- chemical , immunology : Antibodies, Viral, Immune Sera, Vaccines, Inactivated, Viral Vaccines.
- chemical : Aluminum Hydroxide, Propiolactone.
- immunology : SARS Virus.
- Animals, Chlorocebus aethiops, Chromatography, Enzyme-Linked Immunosorbent Assay, Female, Mice, Mice, Inbred BALB C, Vero Cells.
Abstract
We tested the ability of inactivated SARS-CoV vaccine to induce neutralizing antibodies in BALB/c mice. The inactivated vaccine was prepared by SARS-CoV virus propagation in Vero cells, with subsequent beta-propiolactone inactivation and Sepharose 4FF column chromatography purification. One hundred forty BALB/c female mice were divided into seven groups of 20 mice each. Of the seven groups, three groups were inoculated with 0.1, 1, and 3 microg of the vaccine without adjuvant while three other groups were inoculated at the same three dosages of vaccine with aluminum hydroxide as adjuvant, respectively. The remaining group was set up as a blank control. Each mouse was inoculated twice at an interval of 3 weeks. One week after the second immunization, mice sera were collected to detect serum neutralizing antibodies. An assay for determining neutralizing antibody titers was developed. The results can be summarized as follows: (1) higher dosages of vaccine induced higher levels of neutralizing antibody titer; (2) the level of neutralizing antibodies induced by the inoculation with aluminum hydroxide adjuvant was slightly higher than that without adjuvant, but the difference was not statistically significant.
DOI: 10.1089/104454904323145272
PubMed: 15231072
Affiliations:
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induces a high level of neutralizing antibodies in BALB/c mice.</title><author><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Genomics Institute, Chinese Academy of Sciences, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Sciences</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name></author><author><name sortKey="Qin, Ede" sort="Qin, Ede" uniqKey="Qin E" first="Ede" last="Qin">Ede Qin</name></author><author><name sortKey="Yu, Man" sort="Yu, Man" uniqKey="Yu M" first="Man" last="Yu">Man Yu</name></author><author><name sortKey="Ding, Zhifen" sort="Ding, Zhifen" uniqKey="Ding Z" first="Zhifen" last="Ding">Zhifen Ding</name></author><author><name sortKey="Shi, Huiying" sort="Shi, Huiying" uniqKey="Shi H" first="Huiying" last="Shi">Huiying Shi</name></author><author><name sortKey="Cheng, Xiaojie" sort="Cheng, Xiaojie" uniqKey="Cheng X" first="Xiaojie" last="Cheng">Xiaojie Cheng</name></author><author><name sortKey="Wang, Caiping" sort="Wang, Caiping" uniqKey="Wang C" first="Caiping" last="Wang">Caiping Wang</name></author><author><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name></author><author><name sortKey="Zhu, Qiang" sort="Zhu, Qiang" uniqKey="Zhu Q" first="Qiang" last="Zhu">Qiang Zhu</name></author><author><name sortKey="Fang, Fang" sort="Fang, Fang" uniqKey="Fang F" first="Fang" last="Fang">Fang Fang</name></author><author><name sortKey="Chang, Haiyan" sort="Chang, Haiyan" uniqKey="Chang H" first="Haiyan" last="Chang">Haiyan Chang</name></author><author><name sortKey="Li, Shuangli" sort="Li, Shuangli" uniqKey="Li S" first="Shuangli" last="Li">Shuangli Li</name></author><author><name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name></author><author><name sortKey="Chen, Xishu" sort="Chen, Xishu" uniqKey="Chen X" first="Xishu" last="Chen">Xishu Chen</name></author><author><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name></author></analytic><series><title level="j">DNA and cell biology</title><idno type="ISSN">1044-5498</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum Hydroxide</term><term>Animals</term><term>Antibodies, Viral (immunology)</term><term>Chlorocebus aethiops</term><term>Chromatography</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Immune Sera (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Propiolactone</term><term>SARS Virus (immunology)</term><term>Vaccines, Inactivated (immunology)</term><term>Vero Cells</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Cellules Vero</term><term>Chromatographie</term><term>Femelle</term><term>Hydroxyde d'aluminium</term><term>Propiolactone</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Sérums immuns (immunologie)</term><term>Test ELISA</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins inactivés (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immune Sera</term><term>Vaccines, Inactivated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Aluminum Hydroxide</term><term>Propiolactone</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Sérums immuns</term><term>Vaccins antiviraux</term><term>Vaccins inactivés</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Chromatography</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Chromatographie</term><term>Femelle</term><term>Hydroxyde d'aluminium</term><term>Propiolactone</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We tested the ability of inactivated SARS-CoV vaccine to induce neutralizing antibodies in BALB/c mice. The inactivated vaccine was prepared by SARS-CoV virus propagation in Vero cells, with subsequent beta-propiolactone inactivation and Sepharose 4FF column chromatography purification. One hundred forty BALB/c female mice were divided into seven groups of 20 mice each. Of the seven groups, three groups were inoculated with 0.1, 1, and 3 microg of the vaccine without adjuvant while three other groups were inoculated at the same three dosages of vaccine with aluminum hydroxide as adjuvant, respectively. The remaining group was set up as a blank control. Each mouse was inoculated twice at an interval of 3 weeks. One week after the second immunization, mice sera were collected to detect serum neutralizing antibodies. An assay for determining neutralizing antibody titers was developed. The results can be summarized as follows: (1) higher dosages of vaccine induced higher levels of neutralizing antibody titer; (2) the level of neutralizing antibodies induced by the inoculation with aluminum hydroxide adjuvant was slightly higher than that without adjuvant, but the difference was not statistically significant.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name><name sortKey="Chang, Haiyan" sort="Chang, Haiyan" uniqKey="Chang H" first="Haiyan" last="Chang">Haiyan Chang</name><name sortKey="Chen, Xishu" sort="Chen, Xishu" uniqKey="Chen X" first="Xishu" last="Chen">Xishu Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Cheng, Xiaojie" sort="Cheng, Xiaojie" uniqKey="Cheng X" first="Xiaojie" last="Cheng">Xiaojie Cheng</name><name sortKey="Ding, Zhifen" sort="Ding, Zhifen" uniqKey="Ding Z" first="Zhifen" last="Ding">Zhifen Ding</name><name sortKey="Fang, Fang" sort="Fang, Fang" uniqKey="Fang F" first="Fang" last="Fang">Fang Fang</name><name sortKey="Li, Shuangli" sort="Li, Shuangli" uniqKey="Li S" first="Shuangli" last="Li">Shuangli Li</name><name sortKey="Qin, Ede" sort="Qin, Ede" uniqKey="Qin E" first="Ede" last="Qin">Ede Qin</name><name sortKey="Shi, Huiying" sort="Shi, Huiying" uniqKey="Shi H" first="Huiying" last="Shi">Huiying Shi</name><name sortKey="Wang, Caiping" sort="Wang, Caiping" uniqKey="Wang C" first="Caiping" last="Wang">Caiping Wang</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name><name sortKey="Yu, Man" sort="Yu, Man" uniqKey="Yu M" first="Man" last="Yu">Man Yu</name><name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name><name sortKey="Zhu, Qiang" sort="Zhu, Qiang" uniqKey="Zhu Q" first="Qiang" last="Zhu">Qiang Zhu</name><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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